A comment from a referee led me to find a method to describe curvature of membranes. This is a quick write-up of our solution. I couldn’t find a solution readily available in Fiji, so I created one using a combination of Fiji for tracing the curvature and IgorPro to do the fitting. If there is […]
Tag: IgorPro
Line Up: Waffle plots for colocalisation data
Quantifying the degree of colocalisation of two signals in microscopy images is very tricky. Lots has been written on this topic, including in my book The Digital Cell. The focus of this post is on visualising colocalisation. One way to look at colocalisation is two think about two sets of objects and how many of […]
Seles: Tennis coding challenge
A colleague sent me a coding challenge. This is a short post to describe how I tackled it. Edit: a follow-up post is here. This is the challenge: There is a tennis match between two players denoted 1, and 2. You are given a sequence of 1s and 2s whereby each number denotes a point […]
Shiny Cage: visualising clathrin triskelia in a lattice
Clathrin is a three-legged protein complex or triskelion that can assemble into lattice-like structures. Inside the cell, this assembly helps to create vesicles: tiny packages of membranes containing proteins and goodies for the cell to use. Incredibly our first view of assembled clathrin was made in the 1960s, with resolution improving steadily since then. The […]
Crackerblocks: computing solutions for IQ Block game
The IQ Block game is a puzzle where the player must fit eight shapes into a square space. The challenge is to find as many ways as possible to do it. The box says there are more than 40 solutions! So how many are there? I wrote a solver to crack the IQ Block game. […]
Cluster One II: comparing cycling performance on similar courses
A short follow-up post. Previously, I looked at how to reproduce a Strava feature that compares performance over similar courses. With a few modifications to the code, I was able to analyse a much larger dataset of cycling performance on similar courses. Two courses with the highest number of tracks are shown below. I cycle […]
Cluster One: comparing running performance on similar courses
One of several features that Strava put behind a paywall was the ability to compare performance on similar courses. I miss this comparison tool and wondered how hard it would be to code my own. This post is a walkthrough of how I approached the problem. The code is available here. It uses the trackeR […]
Find The Answer Within: using grep to find lost code
A short tech-tip this week. How can you find a line of code somewhere on your computer? I often find that I need to write a line of code and I can’t remember the exact syntax. To add to the frustration, I can remember writing a similar line before, but can’t remember in which file […]
Walk This Way
Over the holidays, I had an idea about looping an animation between two images. I wrote some code to do this in Igor Pro (sorry, no R this time…). This post describes how the code works and how you can make a similar animation. There was a reason to do this animation, but as a […]
Yesterday’s Numbers
A quick post this week. I write “this week” in an attempt to convince regular readers that weekly posting will continue. I noticed that J. Cell Sci. give download metrics for their papers and that these downloads are categorised into abstract, full-text and PDF. I was interested in how one of my papers performed. After […]