In the lab we have been doing quite a bit of analysis of cell migration in 2D. Typically RPE1 cells migrating on fibronectin-coated glass. There are quite a few tools out there to track cell movements and to analyse their migration. Naturally, none of these did quite what we wanted and none fitted nicely into […]
Category: computing
Your Favorite Thing: Algorithmically Perfect Playlist
I’ve previously written about analysing my iTunes library and about generating Smart Playlists in iTunes. This post takes things a bit further by generating a “perfect playlist” outside of iTunes… it is exclusively for nerds. How can you put together a perfect playlist? What are your favourite songs? How can you tell what they are? […]
Tips from the blog VII: Recolour Z-stack and Save Projection
I’m putting this up here in case it is useful for somebody. We capture Z-stacks on a Perkin Elmer Spinning Disk microscope system. I wanted to turn each stack into a single image so that we could quickly compare them. This simple macro does the job. We import the images straight from the *.mvd2 library using […]
Tips from the blog VI: doc to pdf
A while back I made this little Automator script to convert Microsoft Word doc and docx files to PDF. It’s useful for when you are sent a bunch of Word files for committee work. Opening PDFs in Preview is nice and hassle-free. Struggling with Word is not. It’s not my own work, I just put it […]
Green is the Colour: mNeonGreen spectra
I was searching for the excitation and emission spectra for mNeonGreen. I was able to find an image, but no values for the spectra. Here is an approximation of the spectra (xlsx format, still haven’t figured out csv for wordpress). I got these values using IgorThief.ipf a very handy tool that allows the extraction of XY […]
Tips from the blog IV – averaging
I put a recent code snippet put up on the IgorExchange. It’s a simple procedure for averaging a set of 1D waves and putting the results in a new wave. The difference between this code and Average Waves.ipf (which ships with Igor) is that this function takes the average of all points in the wave and […]
Wrong Number: A closer look at Impact Factors
This is a long post about Journal Impact Factors. Thanks to Stephen Curry for encouraging me to post this. tl;dr — I really liked this recent tweet from Stat Fact It’s a great illustration of why reporting means for skewed distributions is a bad idea. And this brings us quickly to Thomson-Reuters’ Journal Impact Factor […]
Waiting to happen II: Publication lag times
Following on from the last post about publication lag times at cell biology journals, I went ahead and crunched the numbers for all journals in PubMed for one year (2013). Before we dive into the numbers, a couple of points about this kind of information. Some journals “reset the clock” on the received date with manuscripts […]
Waiting to Happen: Publication lag times in Cell Biology Journals
My interest in publication lag times continues. Previous posts have looked at how long it takes my lab to publish our work, how often trainees publish and I also looked at very long lag times at Oncogene. I recently read a blog post on automated calculation of publication lag times for Bioinformatics journals. I thought it would […]
Division Day: using PCA in cell biology
In this post I’ll describe a computational method for splitting two sides of a cell biological structure. It’s a simple method that relies on principal component analysis, otherwise known as PCA. Like all things mathematical there are some great resources on the web, if you want to understand this operation in more detail (for example, this great […]