We have a new paper out! You can access it here. Title of the paper: The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle What’s it about? When a cell divides, the two new cells need to get the right number of chromosomes. If this process goes wrong, it […]
Tag: cell biology
Green is the Colour: mNeonGreen spectra
I was searching for the excitation and emission spectra for mNeonGreen. I was able to find an image, but no values for the spectra. Here is an approximation of the spectra (xlsx format, still haven’t figured out csv for wordpress). I got these values using IgorThief.ipf a very handy tool that allows the extraction of XY […]
Zero Tolerance
We were asked to write a Preview piece for Developmental Cell. Two interesting papers which deal with the insertion of amphipathic helices in membranes to influence membrane curvature during endocytosis were scheduled for publication and the journal wanted some “front matter” to promote them. Our Preview is paywalled – sorry about that – but I […]
Waiting to Happen: Publication lag times in Cell Biology Journals
My interest in publication lag times continues. Previous posts have looked at how long it takes my lab to publish our work, how often trainees publish and I also looked at very long lag times at Oncogene. I recently read a blog post on automated calculation of publication lag times for Bioinformatics journals. I thought it would […]
Division Day: using PCA in cell biology
In this post I’ll describe a computational method for splitting two sides of a cell biological structure. It’s a simple method that relies on principal component analysis, otherwise known as PCA. Like all things mathematical there are some great resources on the web, if you want to understand this operation in more detail (for example, this great […]
Sticky End
We have a new paper out! You can access it here. The work was mainly done by Cristina Gutiérrez Caballero, a post-doc in the lab. We had some help from Selena Burgess and Richard Bayliss at the University of Leicester, with whom we have an ongoing collaboration. The paper in a nutshell We found that TACC3 binds […]
Joining A Fanclub
When I started this blog, my plan was to write about interesting papers or at least blog about the ones from my lab. This post is a bit of both. I was recently asked to write a “Journal Club” piece for Nature Reviews Molecular Cell Biology, which is now available online. It’s paywalled unfortunately. It’s […]
Insane In The Brain
Back of the envelope calculations for this post. An old press release for a paper on endocytosis by Tom Kirchhausen contained this fascinating factoid: The equivalent of the entire brain, or a football field of membrane, is turned over every hour If this is true it is absolutely staggering. Let’s check it out. A synaptic […]
Strange Things
I noticed something strange about the 2013 Impact Factor data for eLife. Before I get onto the problem. I feel I need to point out that I dislike Impact Factors and think that their influence on science is corrosive. I am a DORA signatory and I try to uphold those principles. I admit that, in the […]
Round and Round
I thought I’d share a procedure for rotating a 2D set of coordinates about the origin. Why would you want do this? Well, we’ve been looking at cell migration in 2D – tracking nuclear position over time. Cells migrate at random and I previously blogged about ways to visualise these tracks more clearly. Part of […]